Case Proteomics Center Beamlines for Protein Crystallography

Babu Manjasetty, Case Western Reserve University, speaking at Advances in Protein Crystallography 2007.

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Abstract
At National Synchrotron Light Source, Case Proteomics Center (CPC) operates two tunable beamlines suitable for SAD/MAD data collection. The first beamline X3A is located on a bending magnet of the X-ray storage ring. This beamline, and its companion X3B, have been in operation for X-ray crystallography experiments since 2000 and its users have generated X-ray data that has resulted in over 400 PDB deposits since that time. The second beamline X29, #1 beamline in east coast, constructed to promote the high-throughput data collection of huge amount of protein crystals associated with the New York Structural Genomics Research Consortium (NYSGXRC) which utilizes 30% of beamtime through the CCP.

Recent developments at these two beamlines are presented that will enable the facilities to handle of the large numbers of crystals that are coming from the genomics projects. In addition, we will show the successful results several challenging and diverse crystallographic experiments such as phasing of crystals with large number (100+ Se) or small number (1 Se) substructures, crystals with large unit cells (macromolecular assemblies), very small crystals (micro-crystals), very weakly diffracting crystals (membrane proteins). The quality of data and productivity of both the beamlines has resulted in large numbers of peer-reviewed publications in top international journals.

About the Speaker
Babu Manjasetty is a biophysicist, who does research in the areas of protein crystallography structural proteomics. He received his doctoral degree from Mysore University, India. He became a recipient of the Humboldt Research Fellowship Award, Germany. During his post doctoral training, he has solved number of protein structures including, gankyrin, an oncoprotein linked to liver cancer, bifunctional aldolase-dehydrogenase sequestering a reactive and volatile intermediate (DmpFG), Aortic Preferentially Expressed Protein-1 (APEG-1), which plays a in the growth and differentiation of arterial smooth muscle cells. Presently, he works for Case Proteomics Center and engaged in synchrotron based protein crystallography research.

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